- Data Collection:
- Acquiring Data
- My data was to extract the area of the xylem from our newly acquired images. By using the macro that Dr. Weiss came up with to segment the xylem, I used the Analyze Particle tool to automatically measure the area of the xylem. (Analyze>Analyze Particle)
- This tool automatically traced the xylem and gave me the number of pixels in the xylem. The image below shows the manual process of hand tracing the xylem.
- I ended up running a two-tailed, two sample equal variance t-Test. I ran this test because it measures how close the automated process is to the hand traced data set. The more similar the data are to each other, the more accurate the automated process is. The image below is the data set for the three samples
- After running the t-test, the P-value came out to be .916466 which is > .05 meaning that the automated process proves to not only be faster, but can reduce other variables that could affect the data of the manual process.