- Data Collection:
- Dr. Weiss
- Issues before his arrival: Dr. Weiss mentioned that our previous methods of removing the pith was very interesting, however it lacked many components. One of which was using the K-cluster means with the color thresholding and Trainable Weka segmentation. The issue with this was that it did not use color as a segmentation characteristic, which was very crucial to our project.
- Color Thresholding: We used this plugin to focus on removing the xylem, because Dr. Weiss suggested that if we removed that tissue, we could easily remove the pith. This plugin uses Hue Saturation and Brightness as a tool to segment out the xylem. Since the xylem is stained a reddish pink color, we set our Hue to focus on just the red light spectrum. We leave the Saturation and Brightness levels at the absolute maximum and minimum values because that would give us a bolder and more intense red color which highlights the xylem. Example:
- From here we would open the Macro that Dr. Weiss had and change the values in which we placed on the Hue, Saturation, and Brightness. And click run Macro
Results:
Original
Final