Week 6

  1. Data Collection:
  • Color Deconvolution
  • This plugin unmixes an RGB image produced by subtractive mixing (inks, histological dyes) into separate channels corresponding to up to 3 determined colors. This is useful to do pigment separation. In other words it tells you which part is red, which part is blue…
  • What we want to accomplish is to separate the safranin and fast green color within the image. Results:
  • tt1. Trial #1                                               2. Original

 

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3. Final

                2. Summarize Accomplishments and briefly explain next step:

  • Meeting and finally being able to work with Dr. Weiss, has been an incredible experience! He has a lot of knowledge and really helped us refine our basic skills on ImageJ. I was able to recreate what he did with another image and our next plan is to use Dr. Bell’s automated camera in his lab and retake the images using a 10x lens.